flow cytometry software flojo v.10  (Tree Star Inc)

Journal: PLoS Pathogens

Article Title: KSHV requires vCyclin to overcome replicative senescence in primary human lymphatic endothelial cells

doi: 10.1371/journal.ppat.1008634

Figure Lengend Snippet: Early and late passage mock and KSHV-infected BECs and LECs were harvested, fixed, and stained with propidium iodide. Cell cycle was analyzed by flow cytometry. A-D) Representative histograms from early passage (A and C) and late passage (B and D) mock (A and B) and KSHV-infected (C and D) LECs showing percentage of cells in each phase of the cell cycle. E) Quantification of the percentage of cells in each phase of the cell cycle of early and late passage mock- and KSHV-infected BECs from a representative experiment. F) Quantification of the percentage of cells in each phase of the cell cycle of early and late passage mock- and KSHV-infected LECs from a representative experiment. This experiment was performed three times with similar results.

Article Snippet: Data was analyzed using FloJo flow cytometry analysis software (Tree Star, Inc.; Ashland, OR).

Techniques: Infection, Staining, Flow Cytometry

Journal: Blood

Article Title: Interphase Chromosome Flow-FISH

doi: 10.1182/blood-2012-05-434266

Figure Lengend Snippet: Mixing normal donor and monosomy 7 patient cells. Mononuclear cells from an MDS patient with monosomy 7 and from a normal donor were analyzed with IC Flow-FISH independently (without mixing) and after mixing in differing proportions. From flow cytometry data, histograms of chromosome 7 probe fluorescence values were prepared for unmixed patient (A) and unmixed donor (B) monocyte populations. (C-D) Samples having mixtures of patient and donor mononuclear cells, with 5% patient cells expected in panel C and 10% patient cells expected in panel D. Monocytes (cells corresponding to gated regions in scatter plots) were selected, and histograms of monocyte probe fluorescence were prepared as in panels A and B. Proportions of each population were found by applying a 2-component Gaussian mixture model, and the fitted normal curves shown on histograms were generated from the mixture model results. The monosomy 7 populations (lower-binding populations; red normal curve in histograms) were found to represent 21.0% (C) and 38.2% (D) of monocytes.

Article Snippet: Flow cytometry data were acquired and analyzed with FloJo flow cytometry software (Version 8.8.7; TreeStar), and further analysis was performed with the R statistical software package using the mixtools library for analyzing finite mixture models.

Techniques: Flow Cytometry, Fluorescence, Generated, Binding Assay